Amyloid Fibrillation of Bovine Alpha Lactalbumin
نویسندگان
چکیده
منابع مشابه
the inhibitory effects of cuminaldehyde on amyloid fibrillation and cytotoxicity of alpha-synuclein
objective: alpha-synuclein is a major component of protein plaques in synucleinopathies, particularly parkinson’s disease. the purpose of this study is to assess the inhibitory effects of cuminaldehyde on the fibrillation of alpha-synuclein. methods: alpha-synuclein was expressed in escherichia coli and subsequently purified. for the process of fibrillation, purified protein was incubated at 3...
متن کاملComparison of the amino acid sequence of bovine alpha-lactalbumin and hens egg white lysozyme.
hydride, was essentially the same as the release of unlabeled Nacetylgalactosaminitol from sialidase-treated OSM. These results suggest that the 14C-OSP contained the glycose units linked to polypeptide in the same manner as in the native OSM, i.e. to serine or threonine, or both. Numerous attempts to demonstrate this linkage directly by amino acid analysis before and after alkaline borohydride...
متن کاملSelective sulfenylation of tryptophan residues in alpha-lactalbumin of bovine milk.
Tryptophan residues of bovine cr-la&albumin, one of the two protein components of lactose synthetase, were selectively sulfenylated with Z-nitrophenylsulfenyl chloride. When the reaction was performed in 0.1 M acetic acid for 1 hour, a modified protein containing 1 .O nitrophenylsulfenyl tryptophan residue per a-la&albumin molecule was formed and isolated in 42% yield. Upon reduction, carboxyme...
متن کاملMetal ion binding to the N and A conformers of bovine alpha-lactalbumin.
The binding of Ca2+, Zn2+, Tb3+, and Mn2+ to metal-free bovine alpha-lactalbumin (apo-BLA) was studied by both analytical gel filtration using isotopic metal ions and by fluorescence titration. In the absence of other metal ions, Ca2+ binds at a single site on apo-BLA. The pH dependence of pKa for calcium binding indicates that carboxylate groups of aspartic and/or glutamic residues are coordin...
متن کاملGrowth and safety in term infants fed reduced-protein formula with added bovine alpha-lactalbumin.
OBJECTIVE To assess growth, tolerance, and biochemical measures of protein status in term infants fed an experimental formula with reduced total protein concentration and enriched in bovine alpha-lactalbumin prepared from an alpha-lactalbumin dominant bovine whey. METHODS Healthy, term, exclusively formula-fed infants < or =14 days postnatal age, between 10th and 90th percentiles in weight an...
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ژورنال
عنوان ژورنال: Biophysical Journal
سال: 2009
ISSN: 0006-3495
DOI: 10.1016/j.bpj.2008.12.2296